Our Lab uses the state-of-the-art equipment of the UCY Medical School, as well as modern dedicated equipment for our Lab's research applications.
The Lab has the capacity to do research in vitro and in vivo, using mammalian cell lines, bacteria, rodents and zebrafish.
Contents Presented Below
- Lab's research capacity
- Examples of Lab's equipment
Lab's research capacity
- Use of a diverse compound library with experimental compounds, clinical drugs and natural extracts (>800 compounds).
- Use of a diverse bank of healthy mammalian cell lines, cancer cell lines and bacterial species.
- Application of High-Throughput Screenings (1536-well plate format capacity) in various detection modes (absorbance, luminescence, fluorescence, time-resolved fluorescence, fluorescence polarisation, FRET, TR-FRET, Alpha, BRET).
- Operation of assays in various formats (ELISA, AlphaScreen, HTRF).
- Application of various assays and assessment of various endpoints in mammalian cells (cell viability, colony formation, proliferation, metabolic activity, oxidative stress, protein mass, ATP assay, apoptosis/necrosis, cytostasis, DNA damage, invasion/migration, antioxidant efficacy, BBB pass simulation, stress response, enzyme kinetics).
- Application of a portfolio of optimised in vitro bacterial assays (microplate growth inhibition, resazurin turbidometry, agar disk-diffusion, time-kill kinetics, agar dilution, live/dead stain, bacterial membrane stability, mammalian cell infection, kinetic dose accumulation, agar colony forming, multi-drug resistance, persistent population growth).
- Assessment of various endpoints in bacterial cells, such as: Minimum Inhibitory Concentration (MIC), Inhibitory potency (IC50), Minimum Bactericidal Concentration (MBC), Spectrum efficacy (SE), Time-Dependence Activity profile (TDA), Dose-Accumulation Activity profile (DAA), Bacteriostatic activity profile (BA), Metabolic activity profile (MA).
- Pharmacological characterisation of the interaction of compounds with their targets (affinity, efficacy, potency).
- DNA handling (extraction/isolation, cloning/transfection, DNA amplification, plasmid manipulation).
- Application of in vitro toxicological analysis (LD50, toxicokinetics)
- Application of in vivo toxicological studies in Zebrafish eggs, larvae and young fish (LD50, toxicokinetics, fixed-dose procedure, up-down procedure, repeated dosing, subchronic & chronic toxicity, one-generation & two-generation reproduction toxicity, neurotoxicity, developmental toxicity)
- Rodent (mice & rats) behavioural and locomotor analysis in a fully-controlled recordable environment.
- Application of various paradigms in rodent modelling (open-field activity/locomotion, fear conditioning, active avoidance, passive avoidance, panic response, light/dark test, latent inhibition, learned helplessness, place preference)
Examples of Lab's equipment